INIA » Proyecto » Fingerprint of potato germplasm using SSR markers applied in the varieties identification and the potato genetic breeding

Proyecto

Fingerprint of potato germplasm using SSR markers applied in the varieties identification and the potato genetic breeding

Rubro Papa
Dependencia Remehue - Osorno
Duración Desde 01-12-2005 Hasta 31-12-2006
Encargado (a) Boris Sagredo Diaz

Objetivo General

To strengthen the potato germplasm identification in Chile using SSRs technology and to develop cheap and quick protocols of DNA isolation adapted to the national actually.

Objetivos Específicos

  • To establish a unique DNA profile for all the potato varieties described in Chile, using an informative SSRs group in pseudomultiplex PCR condition
  • To design a tissue sampling system adapted to conventional protocol of identification and to improve quick and cheap protocols of DNA isolation.
  • To develop methods of DNA isolation for industry potato products.
  • To adapt a technology for variety mixture detection using for PCR DNA from combined samples of tissue.
  • To compare DNA profiles in a strategic group of the in vitro and in field potato collection to estimate the efficiency of the potato germplasm management in the INIA-Remehue.

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