Proyecto

Equipo de Trabajo

Objetivo General

Desarrollar nuevas tecnologías de mejoramiento genético de vides y frutales de carozo con foco en tolerancia a hongos y resistencia a virus.

Objetivos Específicos

• Implementar tecnología de edición génica en diversos genotipos de Vitis spp. y Prunus spp.
• Generar nuevas líneas frutales mejoradas con tolerancia a hongos y resistencia a virus.
• Evaluar las líneas mejoradas obtenidas.

Publicaciones

1. Synthesis of an Artificial Vitis vinifera miRNA 319e Using Overlapping Long Primers and Its Application for Gene Silencing Álvaro Castro, Daniela Quiroz, Evelyn Sánchez, María de los Ángeles Miccono, Carlos Aguirre, Alejandra Ramírez, Christian Montes, Humberto Prieto J Biotechnol. 2016 Sep 10;233:200-10. doi: 10.1016/j.jbiotec.2016.06.028. Epub 2016 Jul 10. (2016)
2. A draft genome sequence of Pseudomonas veronii R4: a grapevine (Vitis vinifera L.) root-associated strain with high biocontrol potential. Christian Montes, Fabiola Altimira, Hayron Canchignia, Álvaro Castro, Evelyn Sánchez, María Miccono, Eduardo Tapia, Álvaro Sequeida, Jorge Valdés, Paz Tapia, Carolina González, Humberto Prieto. Stand. in Genomic Sci. (2016) 11: 76. doi:10.1186/s40793-016-0198-y (2016)
3. Vergara R, Noriega X, Aravena KS, Prieto H and Pérez Correa FJ (2017). ABA represses the expression of cell cycle genes and may modulate the development of endodormancy in grapevine buds. Front. Plant Sci. 8:812. doi: 10.3389/fpls.2017.00812 (2017)
4. Candidate nematicidal proteins in a new Pseudomonas veronii isolate identified by its antagonistic properties against Xiphinema index Hayron Canchignia, Fabiola Altimira, Christian Montes, Evelyn Sánchez, Eduardo Tapia, María Miccono, Daniel Espinoza, Carlos Aguirre, Michael Seeger, Humberto Prieto Journal of General and Applied Microbiology; doi 10.2323/jgam.2016.07.001 (2016)
5. Chialva C, Muñoz C, Miccono M, Eichler E, Calderon L, Prieto H, Lijavetzky D (2018). Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions. Plant Molecular Biology Reporter DOI: 10.1007/s11105-018-1073-3 https://link.springer.com/article/10.1007%2Fs11105-018-1073-3 (2018)
6. Eriksson D, Kershen D, Nepomuceno A, Pogson B, Prieto H, Purnhagen K, Smyth S, Wesseler J, Whelan A (2018). A comparison of the EU regulatory approach to directed mutagenesis with that of other jurisdictions, consequences for international trade and potential steps forward. New Phytologist. DOI: 10.1111/nph.15627. (2018)
7. Aranzana MJ, Decroocq V, Dirlewanger E, Eduardo I, Gao ZS, Gasic K, Iezzoni A, Jung S, Peace C, Prieto H, Tao R, Verde I, Abbott AG Arús P (2019). Prunus genetics and applications after de novo genome sequencing: achievements and prospects. Hort Res 6:58. DOI 10.1038/s41438-019-0140-8 (2019)

Presentaciones en Congresos

• Avances en desarrollo de biotools de apoyo a breeding convencional. En Cherry Breeding Workshop. 5 de noviembre de 2015. Fund. Chile, organizado por Biofrutales. (2015)
• Humberto Prieto (2017). Nuevas tecnologías de mejoramiento genético: uso de genes de susceptibilidad para la generación de especies frutales con resistencia a fitopatógenos. Encuentro Científico de la Resiliencia en Plantas: Desafíos Globales, Universidad de Talca, Julio 24-25. Talca. (2017)
• Castro A, Quiroz D, Sánchez E, Miccono M, Aguirre C, Montes C, Prieto H. (2007) Synthesis of an artificial Vitis vinifera miRNA 319e using overlapping long primers and its application for gene silencing. XXV Plant and Animal Genome Conference - San Diego, Jan 14th - 18th. Poster #1208. (2017)
• Miccono MA, Madrid G, Aguirre C, Olivares F, Olmedo B, Mora R, Sánchez E, Quiroz D, Prieto H (2018). DNA replicon-mediated genome editing in grapevine using CRISPR/Cas9 and a paired gRNA strategy. Plant Biology, Montreal, Canada, July 14-18. (2018)
• De Lorenzis G, Caramanico L, Olivares F, Prieto H, Scienza A, Pozzi C, Brancadoro L, Failla O (2018). A genome editing approach to study the drought stress tolerance in Vitis spp. XII International Conference on Grapevine Breeding and Genetics, Bordeaux, France, July 15-20. (2018)
• Madrid G, Quiroz D, Melet L, Sánchez E, Olmedo B, Prieto H (2018). One polycistronic amiRNA to target three different genes. Plant Biology 2018, Montreal, Canada, July 14-18. (2018)

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