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Humberto Godofr Prieto Encalada

Humberto Godofr Prieto Encalada
Cargo Investigador(a) – La Platina
Título(s) Bioquímico
Teléfono (2) 2 5779100
Email @

Publicaciones

  1. Humberto Prieto, "La edición de genes vía CRISP-Cas y su incorporación a los programas de mejoramiento vegetal". Conferencia Magistral I, 20 Septiembre, XXVIII Congreso Nacional y VII Congreso Internacional de Fitogenética, fitogenoma y su potencial en la era biotecnologica para la salud y la alimentación. Organizado por la Sociedad Mexicana de Fitogenética A.C. y la Universidad Veracruzana. México, Septiembre 20, 2021.
  2. Vergara R, Olivares F, Olmedo B, Toro C, Muñoz M, Zúñiga C, Mora R, Plantat P, Miccono M, Loyola R, Aguirre C, Prieto H (2021). Gene Editing in Prunus Spp.: The Challenge of Adapting Regular Gene Transfer Procedures for Precision Breeding. En: "Prunus - Recent Advances", Ayzin Küden (ed), IntechOpen, Londres. DOI: 10.5772/intechopen.98843
  3. Acha G, Vergara R, Muñoz M, Mora R, Aguirre C, Muñoz M, Kalazich J, Prieto H (2021). A Traceable DNA-Replicon Derived Vector to Speed Up Gene Editing in Potato: Interrupting Genes Related to Undesirable Postharvest Tuber Traits as an Example. Plants 10(9), 1882; https://doi.org/10.3390/plants10091882
  4. Felipe Olivares, Rodrigo Loyola, Blanca Olmedo, María de los Ángeles Miccono, Carlos Aguirre, Ricardo Vergara, Danae Riquelme, Gabriela Madrid, Philippe Plantat, Roxana Mora, Daniel Espinoza and Humberto Prieto (2021). CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons. Front. Plant Sci., 23 December 2021 | https://doi.org/10.3389/fpls.2021.791030.
  5. Reynaldo Núñez Salazar, Carlos Aguirre, Jorge Soto, Pamela Salinas, Humberto Prieto and Manuel Paneque (2020). Physicochemical Parameters Affecting the Distribution and Diversity of the Water Column Microbial Community in the High-Altitude Andean Lake System of La Brava and La Punta. Microorganisms 8:1181; doi:10.3390/microorganisms8081181
  6. Lisvaneth Medina, Christian Castillo, Ana Liempi, Jesús Guerrero-Muñoz, Maura Rojas-Pirela, Juan Diego Maya, Humberto Prieto and Ulrike Kemmerling (2020). Trypanosoma cruzi and Toxoplasma gondii Induce a Differential MicroRNA Profile in Human Placental Explants. Front. Immunol., 06 November 2020 | https://doi.org/10.3389/fimmu.2020.595250
  7. Dennis Eriksson, Rodomiro Ortiz, Richard G. F. Visser, Juan Antonio Vives-Vallés and Humberto Prieto (2020). Editorial: Leeway to Operate With Plant Genetic Resources. Front. Plant Sci., 24 July 2020. https://doi.org/10.3389/fpls.2020.00911
  8. Angela Ricci, Silvia Sabbadini, Humberto Prieto, Isabel MG Padilla, Chris Dardick, Zhijian Li, Ralph Scorza, Cecilia Limera, Bruno Mezzetti, Margarita Perez-Jimenez, Lorenzo Burgos and Cesar Petri (2020). Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants 2020, 9, 971; doi:10.3390/plants9080971
  9. Felipe Olivares, Ricardo Vergara, Rodrigo Loyola, Blanca Olmedo, Carlos Aguirre, Roxana Mora, Marisol Muñoz, Philippe Plantat, Giovana Acha, Sandra Sopalda, Daniel Espinoza, Humberto Prieto (2020). Stable editing of pathogen susceptibility genes in grapevine by transient expression geminivirus DNA-replicons monitored by GFP. The International CRISPR and Gene Editing Symposium, September 23rd, 2020. https://webinars.liebertpub.com/e/CRISPRSymposium
  10. Diana Marini, Diego Maeso, Antonio Bustamante, Humberto Prieto (2020). Estado del Arte de la Plataforma Regional para la Prevención y Detección Precoz de Enfermedades Cuarentenarias en Frutales en América Latina y el Caribe. Libro del Fondo Regional de Tecnología Agropecuaria (FONTAGRO), número de serie 001. FONTAGRO / Banco Interamericano de Desarrollo, 1300 New York Avenue, NW, Stop W0908, Washington, D.C., 20577. Disponible en https://www.fontagro.org/new/proyectos/sanidad-frutales/es
  11. Ortiz, R., Eriksson, D., Visser, R. G. F., Prieto, H., Vallés, J. A. V., eds. (2020). Leeway to Operate With Plant Genetic Resources. Lausanne: Frontiers Media SA. doi: 10.3389/978-2-88966-008-7
  12. Diana Marini, Diego Maeso, Antonio Bustamante, Humberto Prieto (2020). Estado del Arte de la Plataforma Regional para la Prevención y Detección Precoz de Enfermedades Cuarentenarias en Frutales en América Latina y el Caribe. Libro del Fondo Regional de Tecnología Agropecuaria (FONTAGRO), número de serie 001. FONTAGRO / Banco Interamericano de Desarrollo, 1300 New York Avenue, NW, Stop W0908, Washington, D.C., 20577.
  13. Rubio J, Sánchez E, Tricon D, Montes C, Eyquard JP, Chague A, Aguirre C, Prieto H, Decroocq V (2019). Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA production. BMC Plant Biology. DOI: 10.1186/s12870-019-2047-9 Title:
  14. Prieto H, Miccono M, Aguirre C, Sánchez E, Castro A (2019). Grape Biotechnology: Past, Present, and Future. En: Compendium of plant genomes: The Grape Genome. Cantu D and Walrker A (eds.), Springer International Publishing, Switzerland, ISBN 978-3-030-18600-5, pp 345-363. https://doi.org/10.1007/978-3-030-18601-2_16
  15. Guo-qing Song, Humberto Prieto, Vladimir Orbovic (2019). Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress and Challenges. Frontiers in Plant Sciences. doi: 10.3389/fpls.2019.00226
  16. Aranzana MJ, Decroocq V, Dirlewanger E, Eduardo I, Gao ZS, Gasic K, Iezzoni A, Jung S, Peace C, Prieto H, Tao R, Verde I, Abbott AG Arús P (2019). Prunus genetics and applications after de novo genome sequencing: achievements and prospects. Hort Res 6:58. DOI 10.1038/s41438-019-0140-8
  17. Eriksson D, Kershen D, Nepomuceno A, Pogson B, Prieto H, Purnhagen K, Smyth S, Wesseler J, Whelan A (2018). A comparison of the EU regulatory approach to directed mutagenesis with that of other jurisdictions, consequences for international trade and potential steps forward. New Phytologist. DOI: 10.1111/nph.15627.
  18. Chialva C, Muñoz C, Miccono M, Eichler E, Calderon L, Prieto H, Lijavetzky D (2018). Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions. Plant Molecular Biology Reporter DOI: 10.1007/s11105-018-1073-3 https://link.springer.com/article/10.1007%2Fs11105-018-1073-3
  19. Humberto Prieto (2018). Nueva era en el mejoramiento genético vegetal: el gran mundo de los RNAs pequeños. LXIII Reunión del Programa Cooperativo Centroamericano para el Mejoramiento de Cultivos y Animales (PCCMCA). Conferencia Inaugural. Ciudad de Panamá, 23 - 27 de abril. Panamá
  20. Miccono MA, Madrid G, Aguirre C, Olivares F, Olmedo B, Mora R, Sánchez E, Quiroz D, Prieto H (2018). DNA replicon-mediated genome editing in grapevine using CRISPR/Cas9 and a paired gRNA strategy. Plant Biology, Montreal, Canada, July 14-18.
  21. De Lorenzis G, Caramanico L, Olivares F, Prieto H, Scienza A, Pozzi C, Brancadoro L, Failla O (2018). A genome editing approach to study the drought stress tolerance in Vitis spp. XII International Conference on Grapevine Breeding and Genetics, Bordeaux, France, July 15-20.
  22. Madrid G, Quiroz D, Melet L, Sánchez E, Olmedo B, Prieto H (2018). One polycistronic amiRNA to target three different genes. Plant Biology 2018, Montreal, Canada, July 14-18.
  23. Prieto H (2018). RNAi y CRISPR-Cas: Nuevas tecnologías de mejoramiento vegetal basadas en RNAs pequeños. Charla Inaugural 1er Simposio Internacional de Biotecnología, Recursos Genéticos y Sustentabilidad en las Ciencias Agropecuarias. Facultad de Ciencias Biológicas y Agropecuarias. Región Orizaba-Córdoba, Universidad Veracruzana. 20 Noviembre de 2018, Veracruz, México.
  24. Prieto H (2018). Grupo NBTs Chile. I Curso Regional de Edición Génica en Plantas y Animales. 30 de noviembre, INTA Balcarce, Balcarce, Argentina.
  25. Olivares F, Prieto H (2018). Small RNAS: Big technologies. Workshop in strategies for sustainable agrifood systems and natural resources in Chile and Sweden. Campus Antumapu, Universidad de Chile, Santiago, 22 de Noviembre de 2018.
  26. Flores-Ortiz C, Miccono M, Madrid G, Correa G, Prieto H, Stange C (2018). USE OF NEW BREEDING TECHNOLOGIES (NBT) BASED IN CRISPR/CAS9 APPROACH TO DEVELOP NEW APPLE VARIETIES WITH ENHANCED ANTIOXIDANT AND PRO-VITAMIN A CAPACITY. XIII Reunión de Biología Vegetal Chile, Puerto Varas, Chile, 3-6 de Diciembre 2018.
  27. Paola Barba, Aurélie Chague, Veronique Decroocq, Jean-Philippe Eyquard, Christian Montes, Humberto Prieto, Daniela Quiroz, Julia Rubio, Evelyn Sanchez and David Tricon. Plum pox virus – Prunus spp. interaction: the small RNA talk. January 2017 · XXV Plant and Animal Genome Conference - Fruits and Nuts Workshop., San Diego, Jan 14th. Pacific Salon 4.; 01/2017
  28. Castro A, Quiroz D, Sánchez E, Miccono M, Aguirre C, Montes C, Prieto H. (2007) Synthesis of an artificial Vitis vinifera miRNA 319e using overlapping long primers and its application for gene silencing. XXV Plant and Animal Genome Conference - San Diego, Jan 14th - 18th. Poster #1208.
  29. Evelyn Sánchez, Karin Rothkegel, Christian Montes, Paola Andrade, Pablo Cid, Andrea M. Almeida and Humberto Prieto. Dormancy-associated microRNAs in sweet cherry (Prunus avium L.): a first draft. January 2017 · XXV Plant and Animal Genome Conference - San Diego, Jan 14th - 18th. Poster 1224
  30. Karin Rothkegel, Evelyn Sanchez, Christian Montes, Sebastián Tapia, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida (2017). DNA methylation and small interference RNAs participate in the regulation of MADS-box genes involved in dormancy in sweet cherry (Prunus avium L.). Tree Physiology. DOI: 10.1093/treephys/tpx055
  31. Cid P, Aguirre C, Sánchez M, Zamorano D, Mihoc M, Salazar E, Chacón G, Navarrete H, Rosas M, Prieto H (2017) An Internet-based platform for the estimation of outcrossing potential between cultivated and Chilean vascular plants. Ecol Evol. 2017;00:1–9. https://doi.org/10.1002/ece3.2854
  32. Sánchez E, Tricon D, Mora R, Quiroz D, Decroocq V, Prieto H (2017). A fast and efficient protocol for small RNA extraction in Japanese plum and other Prunus species. Electronic Journal of Biotechnology, https://doi.org/10.1016/j.ejbt.2017.10.006.
  33. Vergara R, Noriega X, Aravena KS, Prieto H and Pérez Correa FJ (2017). ABA represses the expression of cell cycle genes and may modulate the development of endodormancy in grapevine buds. Front. Plant Sci. 8:812. doi: 10.3389/fpls.2017.00812
  34. Esteban Soto, Evelyn Sánchez, Karin Rothkegel, Christian Montes, Andréa Miyasaka Almeida and Humberto Prieto (2017). Validation of miRNAs involved in regulation of gene expression associated with dormancy and cold requirement in sweet cherry (Prunus avium). VIII International Cherry Symposium, Yamagata, Japan, June 5-9.
  35. Evelyn Sánchez, Paola Andrade, Roxana Mora, Sebastián Godoy, Antonia Yarur, Álvaro Castro, Andréa Miyasaka, and Humberto Prieto (2017). Genetic transformation of sweet cherry cultivars and root-stocks using sweet cherry Flowering locus T gene-expressing and Terminal flower 1 gene-silencing constructs: phenotype evaluation of transgenic trees. VIII International Cherry Symposium, Yamagata, Japan, June 5-9.
  36. Javier Cáceres, Karin Rothkegel, Evelyn Sánchez, Tomás Carrasco, Claudio Meneses, Humberto Prieto and Andréa Miyasaka (2017). A draft genome of Prunus avium cv Karina as a tool for genomic studies. VIII International Cherry Symposium. Yamagata, Japan, June 5-9
  37. Humberto Prieto (2017). Nuevas tecnologías de mejoramiento genético: uso de genes de susceptibilidad para la generación de especies frutales con resistencia a fitopatógenos. Encuentro Científico de la Resiliencia en Plantas: Desafíos Globales, Universidad de Talca, Julio 24-25. Talca.
  38. Godoy S, Tapia E, Seit P, Andrade D, Sánchez E, Andrade P, Miyasaka Almeida A, Prieto H (2017). Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality. In Vitro Cell. Dev. Biol. - Plant. DOI 10.1007/s11627-017-9856-z
  39. Candidate nematicidal proteins in a new Pseudomonas veronii isolate identified by its antagonistic properties against Xiphinema index Hayron Canchignia, Fabiola Altimira, Christian Montes, Evelyn Sánchez, Eduardo Tapia, María Miccono, Daniel Espinoza, Carlos Aguirre, Michael Seeger, Humberto Prieto Journal of General and Applied Microbiology; doi 10.2323/jgam.2016.07.001
  40. Small RNA sequencing and DNA methylation analysis in floral bud reveal that RNA directed DNA Methylation (RdDM) participates during cold accumulation and dormancy release in sweet cherry (Prunus avium L.) Karin Rothkegel, Evelyn Sanchez, Soraya Bravo, Humberto Prieto, Andréa Miyasaka Almeida. Roseaceae Genome Conference 8, Angers, Francia, Junio 2016
  41. Development and evaluation of a temporary immersion system for mass propagation of sweet cherry cultivars and cherry rootstocks. Sebastián Godoy, Paula Seit, Diego Andrade, Andréa Miyasaka, Evelyn Sánchez, Paola Andrade, Humberto Prieto Roseaceae Genome Conference 8, Angers, Francia, Junio 2016.
  42. Synthesis of an Artificial Vitis vinifera miRNA 319e Using Overlapping Long Primers and Its Application for Gene Silencing Álvaro Castro, Daniela Quiroz, Evelyn Sánchez, María de los Ángeles Miccono, Carlos Aguirre, Alejandra Ramírez, Christian Montes, Humberto Prieto J Biotechnol. 2016 Sep 10;233:200-10. doi: 10.1016/j.jbiotec.2016.06.028. Epub 2016 Jul 10.
  43. A draft genome sequence of Pseudomonas veronii R4: a grapevine (Vitis vinifera L.) root-associated strain with high biocontrol potential. Christian Montes, Fabiola Altimira, Hayron Canchignia, Álvaro Castro, Evelyn Sánchez, María Miccono, Eduardo Tapia, Álvaro Sequeida, Jorge Valdés, Paz Tapia, Carolina González, Humberto Prieto. Stand. in Genomic Sci. (2016) 11: 76. doi:10.1186/s40793-016-0198-y
  44. Avances en desarrollo de biotools de apoyo a breeding convencional. En Cherry Breeding Workshop. 5 de noviembre de 2015. Fund. Chile, organizado por Biofrutales.
  45. Outcrossing potential between 11 important genetically modified crops and the Chilean vascular flora. Miguel A. Sánchez, Pablo Cid, Humberto Navarrete, Carlos Aguirre, Gustavo Chacón, Erika Salazar, Humberto Prieto. Plant Biotechnol. J. doi: 10.1111/pbi.12408
  46. Differential RNAi responses of Nicotiana benthamiana individuals transformed with a hairpin-inducing construct during Plum pox virus challenge. Montes C1, Castro A, Barba P, Rubio J, Sánchez E, Carvajal D, Aguirre C, Tapia E, Dell Orto P, Decroocq V, Prieto H. Virus Genes. 2014 Oct;49(2):325-38. doi: 10.1007/s11262-014-1093-5. Epub 2014 Jun 26.
  47. Rubio, J.; Montes, C.; Castro, A.; Álvarez, C.; Olmedo, B.; Muñoz, M.; Tapia, E.; Reyes, F.; Ortega, M.; Sánchez, E.; Miccono, M.; Dalla Costa, L.; Martinelli, L.; Malnoy, M.; Prieto, H. (2014). Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterizationof the best performing individuals in a ?eld trial. Transgenic Research. doi: 10.1007/s11248-014-9811-2
  48. DETERMINATION OF THE DNA METHYLATION AND EXPRESSION KINETIC OF DORMANCY ASSOCIATED MADS-BOX (DAM) GENES IN SWEET CHERRY (Prunus avium) DURING BUD DORMANCY RELEASE Karin Rothkegel, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida. Novena Reunión de Biología Vegetal, 1 al 4 de Diciembre del 2014, La Serena, Región de Coquimbo, Chile.
  49. DETERMINATION OF DNA METHYLATION AND HISTONE MODIFICATION KINETIC IN THE PROMOTER REGION OF DORMANCY ASSOCIATED MADSBOX GENE (DAM6) IN SWEET CHERRY (Prunus avium) DURING BUD DORMANCY RELEASE. Karin Rothkegel, Sebastián Tapia, José Pablo Gonzalez-Vega, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida. 7th International Rosaceae Genomics Conference, Seattle, Washington, USA, June 24-26, 2014.
  50. Pablo Zamora, Ariel Pardo, Angelica Fierro, Humberto Prieto, Gustavo E. Zúñiga. 2013. Molecular characterization of the chalcone isomerase gene family in Deschampsia antarctica. Polar Biology, 36:1269-1280.
  51. Mª Angeles Miccono, Evelyn Sánchez, Alejandra Ramírez, Blanca Olmedo, Patricia Rebufel and Humberto Prieto. The promoter of an ankyrin-like protein from Vitis vinifera is highly induced by Botrytis cinerea infection. Plant Animal Genome (PAG) Conference XX, San Diego, California. 12-16 Enero 2012.
  52. Hayron Canchignia, Michael Seeger,  Myriam Gonzalez, Álvaro Castro,  Eduardo Tapia,  Luis Ortega and Humberto Prieto. Isolation and characterization of new Pseudomonas fluorescens isolates with antagonistic activity against the vector of the Grapevine Fanleaf Virus. Plant Animal Genome (PAG) Conference XX, San Diego, California. 12-16 Enero 2012.
  53. Marcelo Ortega, Christian Montes, Evelyn Sanchez, Humberto Prieto and Gustavo Zuñiga. Deschampsia antarctica dihydroflavonol-4-reductase gene improves salt adaptation in tobacco. Plant Animal Genome (PAG) Conference XX, San Diego, California, enero 12-16, 2012.
  54. Eduardo Tapia, Christian Montes, Patricia Rebufel, Alberto Paradela, Gloria Arenas and Humberto Prieto. Expression of an optimized Argopecten purpuratus antimicrobial peptide in E. coli and evaluation of the purified recombinant protein by in vitro challenges against important plant pathogens. Plant Animal Genome (PAG) Conference XX, San Diego, California, enero 12-16, 2012.
  55. Evelyn Sanchez, Manuel Acuña, Julia Rubio, Paola Barba, Alvaro Castro, Carolina Toro, Andrea Miyasaka de Almeida and Humberto Prieto. Development of transgenic plums using a siRNA construct for improving Plum Pox Virus resistance. Plant and Animal Genome XX Conference, San Diego California, enero 12-16, 2012.
  56. Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor. Álvaro Sequeida, Eduardo Tapia, Marcelo Ortega, Pablo Zamora, Álvaro Castro, Christian Montes, Gustavo E. Zúñiga and Humberto Prieto. (2012) , Electronic Journal of Biotechnology 15(4): Issue of July 15, DOI: 10.2225/vol15-issue4-fulltext-7
  57. Genetic transformation in fruit crops. 6th International Crop Science Congress, Bento Goncalves, Porto Alegre, Brasil, August 10th 2012.
  58. Isolation and characterization of new Pseudomonas fluorescens isolates with antagonistic activity against the vector of the Grapevine Fanleaf Virus. VI Reunión de Biología Vegetal 30 de noviembre - 2 de diciembre, 2011, Pucón, Chile.
  59. Heterologous expression of a grapevine artificial microRNA. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011. Pucón, Chile
  60. Development of a new strategy for silencing of gene-isoforms in Vitis vinifera L. using artificial micro-RNAs. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011, Pucón, Chile
  61. Development of transgenic plums using a siRNA construct for improving Plum Pox Virus resistance. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011 Pucón, Chile.
  62. Green Fluorescent Protein expression directed by the 35S Cauliflower Mosaic Virus promoter in transgenic peach trees. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011, Pucón, Chile
  63. GENETIC TRANSFORMATION OF GRAPE ROOTSTOCKS TO INCREASE SODIUM TOLERANCE. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011, Pucón, Chile
  64. CLONING AND PARTIAL CHARACTERIZATION OF TWO PUTATIVE SODIUM TRANSPORTERS FROM VITIS VINIFERA CV. THOMPSON SEEDLESS. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011, Pucón, Chile.
  65. EXPRESSION OF AN OPTIMIZED ARGOPECTEN PURPURATUS ANTIMICROBIAL PEPTIDE IN E. COLI AND EVALUATION OF THE PURIFIED RECOMBINANT PROTEIN BY IN VITRO CHALLENGES AGAINST IMPORTANT PLANT FUNGI. VI Reunión de Biología Vegetal 30 de noviembre ? 2 de diciembre, 2011, Pucón, Chile.
  66. Genetic Transformation of Vitis spp. as a Tool for the Development and Evaluation of New Technical Platforms, 2010 Pierce?s Disease Research Symposium, December 15 ? 17, San Diego, CA.
  67. Tapia, E., Montes, C., Rebufel, P., Paradela, A., Prieto, H. and Arenas, G. (2011). Expression of an optimized Argopecten purpuratus antimicrobial peptide in E. coli and evaluation of the purified recombinant protein by in vitro challenges against important plant fungi. Peptides doi:10.1016/j.peptides.2011.07.007.
  68. Genetic Transformation Strategies in Fruit Crops
  69. Transformación genética en frutales de vides y carozos en Chile. FRUITTRADE 2011. Hotel Sheraton, Santiago, 28-29 de Septiebre 2011.
  70. Líneas de trabajo en torno a la Vid y la Uva.
  71. Pablo Zamora, Susana Rasmussen, Ariel Pardo, Humberto Prieto and Gustavo E. Zúñiga. cAntioxidant responses of in vitro shoots of Deschampsia antarctica to Polyethylene glycol treatment. Antarctic Science (2010), 22:163-169
  72. Characterization of an ankyrin-like protein up-stream region from Vitis vinifera is highly induced by Botrytis cinerea infection. 10th International Conference on Grapevine Breeding and Genetics, August 01 - 05, 2010, Geneva, New York.
  73. Multifactorial analysis of fungal tolerance in genetically modified grapevine plants: defining prototypes for commercial purposes. 10th International Conference on Grapevine Breeding and Genetics, August 01 - 05, 2010, Geneva, New York.
  74. Engineering the Prunus translation initiation factors in stone fruit trees for resistance to Plum pox virus. International Symposium on Plum pox virus, September 5- 9, 2010, Sofia - Bulgaria.
  75. EVALUATION OF THE RESISTANCE OF TRANSGENIC C5 PLUM (Prunus domestica) AGAINST FOUR CHILEAN PLUM POX VIRUS ISOLATES THROUGH MICRO-GRAFTING. Wendy Wong , Paola Barba , Catalina Álvarez, Álvaro Castro , Manuel Acuña, Pablo Zamora, Marlene Rosales, Paola Dell´Orto , Michael R. Moynihan, Ralph Scorza and Humberto Prieto
  76. GENERACIÓN DE NUEVAS VARIEDADES CON TOLERANCIA A FITOPATÓGENOS UTILIZANDO INGENIERÍA GENÉTICA: ESTRATEGIAS ACTUALES EN CHILE,XIX CONGRESO SOCHIFIT 2010, 9-12 de Noviembre, PUCÓN - CHILE.

Desarrollo Tecnológico

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  6. 10/03/2011 - METODO DE DETECCION DE HIBRIDOS TRIPLOIDES DE BRASSICA SP. MEDIANTE LA REALIZACION DE PCR UTILIZANDO COMO TEMPLADO DNA OBTENIDO DE INDIVIDUOS HIBRIDOS INTERESPECIFICOS DE CAMPO Y DOS JUEGOS DE PARTIDORES.
    Sistema de detección por PCR de híbridos naturales del género Brassica útil para detectar flujo génico entre especies comerciales, transgénicas y silvestres.

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