Proyecto

Equipo de Trabajo

Objetivo General

Identificar genes claves que determinan la variación de color en frutos de cerezos considerando tanto su aporte a QTL de color y niveles de expresión de estos genes, respectivamente, combinado estrategias de genómica funcional, estructural y comparativa.

Objetivos Específicos

• 1.Identificar genes candidatos asociados al desarrollo de color de fruta en cerezo, a través de análisis de QTLs y RNAseq en poblaciones segregantes, incluyendo bases de datos públicas.
• 2.Establecer los perfiles de expression de los genes claves de color de fruto entre individuos de de dos poblaciones segregantes experimentales.
• 3.Establecer el patrón de segregación de los alelos variantes-ORF significantes de los genes candidatos.
• 4.Determinar el efecto de mejoramiento de modelos genéticos de QTLs, considerando datos de alelos de variantes ORF y expresión de sus genes, respectivos.

Publicaciones

1. Bastías A, Correa F, Rojas P, Martin C, Pérez-Diaz J, Yáñez C, Cuevas M, Verdugo R, Sagredo B (2019) Draft genome sequence data of maqui (Aristotelia chilensis) and identification of SSR markers. Data in Brief 27:104545. doi:https://doi.org/10.1016/j.dib.2019.104545 (2019)
2. Villar L, Lienqueo I, Llanes A, Rojas P, Perez J, Correa F, et al. (2020) Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. cv. Bing). PLoS ONE 15(3): e0230110. https://doi.org/10.1371/journal.pone.0230110 (2020)
3. Bastias A, Oviedo K, Almada R, Correa F, Sagredo B (2020) Identifying and validating housekeeping hybrid Prunus spp. genes for root gene-expression studies. PLoS ONE 15(3): e0228403. https://doi.org/10.1371/journal.pone.0228403 (2020)

Presentaciones en Congresos

• Donoso J.M., Lemus G., Almada R., Perez J., Bastias A., Rojas P., Martin C., Correa F., Sagredo B. Characterization of Chilean sweet cherry (Prunus avium L.) germplasm by means of SSR associated to color, fruit size, maturity time and cross compatibility. XI Reunión de Biología Vegetal, Chillán, Chile, 28 nov- 1 dic. 2016. (2016)
• Sagredo, B., Donoso, J., Lemus, G., Almada, R., Perez, J., Bastias, A., Rojas, P., Martin, C., Correa, F. 2016. Molecular tools for Prunus sp.(L.) improvement. XI Reunión de Biología Vegetal 28 nov al 1 Dic 2016. Presentación Oral (2016)
• Lienqueo I, Villar L, Correa F, Sagredo B, Rojas P, Almada R. 2017.TRANSCRIPTIONAL RESPONSE IN ROOT OF CHERRY ROOTSTOCK MXM60 (Prunus avium x P. mahaleb) UNDER DROUGHT STRESS USING RNA SEQUENCING. XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Villar L, Lienqueo I, Sagredo B, Rojas P, Perez J, Almada R,.2017.MOLECULAR CHARACTERIZATION OF HYPOXIA INDUCED GENE DOMAIN (HIGD) GENES IN Prunus ROOTSTOCKS UNDER HYPOXIA AND OTHER ABIOTIC STRESSES. XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile. (2017)
• Almada R, Villar L, Lienqueo I, Rojas P, Sagredo B, Correa F. 2017. MOLECULAR CHARACTERIZATION OF CONSTANS-LIKE GENES IN SWEET CHERRY (Prunus avium L.) FLOWER BUDS AND LEAVES.XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Correa F, Pérez-Díaz J, Almada R, Rojas P, Torres C, Paneque M, Sagredo B, Bastias A.2017. GENOME IDENTIFICATION AND TRANSFERABILITY OF MICROSATELLITE MARKERS BETWEEN ATRIPLEX SPECIES. XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Alarcón J, Correa F, Pérez- Diaz J, Almada R, Rojas P, Torres C, Paneque M, Bastias A, Sagredo B.2017. GENETIC DIVERSITY OF SOME ATRIPLEX SPECIES AL ESTACIÓN EXPERIMENTAL LAS CARDAS, UNIVERSIDAD DE CHILE (IV REGION). XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Rojas, P., Almada, R., Bastías, A., Pérez, J.L., Sagredo, B. 2017. Prospección de virus en huertos productivos de frambuesa (Rubus Idaeus) en la zona central de Chile. Congreso de la Sociedad Chilena de Fitopatología. Chillán, Región del Bio-Bio. 2-5 Octubre 2017 (2017)
• Sagredo B, Donoso J, Lemus G, Almada R, Bastias A, Pérez J, Rojas P, Pozo F, Correa F. A CHILEAN CORE COLLECTION FOR SWEET CHERRY (Prunus avium L.) BREEDING BY THE MEANS OF DIVERSITY AND FRUIT QUALITY MOLECULAR MARKERS.XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Correa F, Martin C, Bastia A, Perez J, Contreras C, Jana C, Almada R, Rojas P, Verdugo R, Sagredo B, Yáñez C, Cuevas M.2017.THE FIRST DRAFT OF THE SWEET CUCUMBER (Solanum muricatum, AITON) GENOME SEQUENCE. XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Correa F, Martin C, Bastias A, Pérez J, Almada R, Rojas P, Verdugo R, Yánez C, Cuevas M, Sagredo B. 2017. FIRST DRAFT GENOME SEQUENCES OF Prunus SP ROOTSTOCKS OBTAINED BY THE MEANS OF DE NOVO ASSEMBLING.XII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 4th – 7th, 2017. Villarrica. Chile (2017)
• Sagredo B, Donoso J.M., Lemus G, Almada R, Perez J, Bastias A, Rojas P, Pozo F, Correa F. 2018. A Chilean Germplasm Core Collection for Sweet Cherry (Prunus avium L.) breeding by the means of SSR markers.9th International Rosaceae Genomics Conference. June 26-30, 2018Nanjing, China. (2018)
• Correa F., Bastias A., Perez-Diaz J., Pozo F., Beltrán F., Verdugo R. and Sagredo B. (2019).Plataforma Genómica para el estudio de especies locales de interés agronómico: Maqui (Aristotelia chilensis Mol.) y Pepino Dulce (Solanum muricatum Aiton). XLIII annual meeting of the Chilean Biochemistry and Molecular Biology Society (SBBMCh)-XIV annual meeting of Chilean Society of Plant Biologist (CSPB). Hotel Gavina-Iquique, October 8 to 11, 2019. (2019)
• Jorge Pérez-Díaz, Rubén Almada, Francisco Correa, Adriana Bastías, Pamela Rojas and Boris Sagredo. 2017. Characterization of a Hexokinase-like gene induced by root hypoxia in Prunus rootstocks. Plant Biology Conference of American Society of Plant Biologists. 24-28 Junio 2017, Honolulu, Hawaii, USA (2017)
• SEPULVEDA N., VILLAR L., LIENQUEO I., CORREA F., PEREZ-DIAZ J., ROJAS P., DONOSO JM, SAGREDO B., ALMADA R. 2018. TRANSCRIPTOME ANALYSIS OF SWEET CHERRY (PRUNUS AVIUM L.) FLORAL BUDS REVEALS THE ROLE OF MADS-BOX TRANSCRIPTION FACTORS IN THE DIFFERENTIATION PROCESS. XVI Congreso Latinoamericano de Fisiología Vegetal. Cordoba, Argentina, 11-15-Nov 2018 (2018)
• Sepulveda N., Villar L., Lienqueo I., Correa F., Perez-Diaz J., Sagredo B., Rojas P., Donoso J., Almada R. 2018. A GLOBAL VIEW OF TRANSCRIPTOME DYNAMICS DURING FLORAL TRANSITION IN SWEET CHERRY (Prunus avium L.). XIII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 3th – 6th, 2018. Puerto Varas. Chile (2018)
• Villar L., Contardo C., Lienqueo I., Rojas P., Sagredo B., Almada R. 2018. CHARACTERIZATION OF THE EXPRESSION OF LEAFY GENE OF SWEET CHERRY (Prunus avium L.) AND ANALYSIS OF ITS PROMOTER IN TRANSGENIC PLANTS. XIII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 3th – 6th, 2018. Puerto Varas. Chile (2018)
• Lienqueo I., Masciarelli O., Luna V., Villar L., Almada R., Sagredo B., Rojas P. 2018.FLORAL BUD DEVELOPMENT IS RELATED TO DIFFERENT PHYTOHORMONE PROFILES IN THE SWEET CHERRY TREE Prunus avium L..XIII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 3th – 6th, 2018. Puerto Varas. Chile (2018)
• Kuhn N., Ponce C., Flores Y., Molina N., Time A., Sagredo B., Meisel L., Donoso JM. 2018.CHARACTERIZATION OF THE PHYSIOLOGICAL EFFECT OF EXOGENOUSLY APPLIED ABSCISIC ACID ON FOUR SWEET CHERRY VARIETIES. XIII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 3th – 6th, 2018. Puerto Varas. Chile (2018)
• Pizarro M., Sagredo B., Infante R., Contador L., Muñoz C., Donoso JM., Almada R., and Lemus G. 2018. GENOTYPE AND PHENOTYPE CHARACTERIZATION OF A CHERRY COLLECTION (Prunus avium L.), EMPHASIS ON COLOUR AND TEXTURE FRUIT.XIII CHILEAN SOCIETY OF PLANT BIOLOGY MEETING December 3th – 6th, 2018. Puerto Varas. Chile (2018)

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