Proyecto

Equipo de Trabajo

Objetivo General

Desarrollar una plataforma de transformación genética de cerezos para resolver problemas no resueltos por el mejoramiento genético convencional y para verificar la función de genes candidatos identificados mediante genómica

Objetivos Específicos

• Desarrollar protocolos de transformación genética del cerezo a partir de tejido embrionario y somático
• Explorar la posibilidad de usar sistemas de embriogénesis somática en sistemas automatizados de manera de generar líneas transgénicas en cantidad suficiente como para que sean útiles para el desarrollo de nuevas variedades
• Desarrollar y evaluar nuevos vectores de transformación genética con énfasis en cisgenia e intragenia utilizando secuencias homólogas
• Utilizar la plataforma desarrollada tanto para la evaluación de la función génica de genes candidatos como para la producción de líneas transgénicas útiles para el mejoramiento genético

Publicaciones

1. Godoy S, Tapia E, Seit P, Andrade D, Sánchez E, Andrade P, Miyasaka Almeida A, Prieto H (2017). Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality. In Vitro Cell. Dev. Biol. - Plant. DOI 10.1007/s11627-017-9856-z (2017)
2. Differential RNAi responses of Nicotiana benthamiana individuals transformed with a hairpin-inducing construct during Plum pox virus challenge. Montes C1, Castro A, Barba P, Rubio J, Sánchez E, Carvajal D, Aguirre C, Tapia E, Dell Orto P, Decroocq V, Prieto H. Virus Genes. 2014 Oct;49(2):325-38. doi: 10.1007/s11262-014-1093-5. Epub 2014 Jun 26. (2014)
3. Karin Rothkegel, Evelyn Sanchez, Christian Montes, Sebastián Tapia, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida (2017). DNA methylation and small interference RNAs participate in the regulation of MADS-box genes involved in dormancy in sweet cherry (Prunus avium L.). Tree Physiology. DOI: 10.1093/treephys/tpx055 (2017)
4. Sánchez E, Tricon D, Mora R, Quiroz D, Decroocq V, Prieto H (2017). A fast and efficient protocol for small RNA extraction in Japanese plum and other Prunus species. Electronic Journal of Biotechnology, https://doi.org/10.1016/j.ejbt.2017.10.006. (2017)

Presentaciones en Congresos

• Small RNA sequencing and DNA methylation analysis in floral bud reveal that RNA directed DNA Methylation (RdDM) participates during cold accumulation and dormancy release in sweet cherry (Prunus avium L.) Karin Rothkegel, Evelyn Sanchez, Soraya Bravo, Humberto Prieto, Andréa Miyasaka Almeida. Roseaceae Genome Conference 8, Angers, Francia, Junio 2016 (2016)
• Development and evaluation of a temporary immersion system for mass propagation of sweet cherry cultivars and cherry rootstocks. Sebastián Godoy, Paula Seit, Diego Andrade, Andréa Miyasaka, Evelyn Sánchez, Paola Andrade, Humberto Prieto Roseaceae Genome Conference 8, Angers, Francia, Junio 2016. (2016)
• Esteban Soto, Evelyn Sánchez, Karin Rothkegel, Christian Montes, Andréa Miyasaka Almeida and Humberto Prieto (2017). Validation of miRNAs involved in regulation of gene expression associated with dormancy and cold requirement in sweet cherry (Prunus avium). VIII International Cherry Symposium, Yamagata, Japan, June 5-9. (2017)
• Evelyn Sánchez, Paola Andrade, Roxana Mora, Sebastián Godoy, Antonia Yarur, Álvaro Castro, Andréa Miyasaka, and Humberto Prieto (2017). Genetic transformation of sweet cherry cultivars and root-stocks using sweet cherry Flowering locus T gene-expressing and Terminal flower 1 gene-silencing constructs: phenotype evaluation of transgenic trees. VIII International Cherry Symposium, Yamagata, Japan, June 5-9. (2017)
• Javier Cáceres, Karin Rothkegel, Evelyn Sánchez, Tomás Carrasco, Claudio Meneses, Humberto Prieto and Andréa Miyasaka (2017). A draft genome of Prunus avium cv Karina as a tool for genomic studies. VIII International Cherry Symposium. Yamagata, Japan, June 5-9 (2017)
• Evelyn Sanchez, Manuel Acuña, Julia Rubio, Paola Barba, Alvaro Castro, Carolina Toro, Andrea Miyasaka de Almeida and Humberto Prieto. Development of transgenic plums using a siRNA construct for improving Plum Pox Virus resistance. Plant and Animal Genome XX Conference, San Diego California, enero 12-16, 2012. (2012)
• DETERMINATION OF THE DNA METHYLATION AND EXPRESSION KINETIC OF DORMANCY ASSOCIATED MADS-BOX (DAM) GENES IN SWEET CHERRY (Prunus avium) DURING BUD DORMANCY RELEASE Karin Rothkegel, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida. Novena Reunión de Biología Vegetal, 1 al 4 de Diciembre del 2014, La Serena, Región de Coquimbo, Chile. (2014)
• DETERMINATION OF DNA METHYLATION AND HISTONE MODIFICATION KINETIC IN THE PROMOTER REGION OF DORMANCY ASSOCIATED MADSBOX GENE (DAM6) IN SWEET CHERRY (Prunus avium) DURING BUD DORMANCY RELEASE. Karin Rothkegel, Sebastián Tapia, José Pablo Gonzalez-Vega, Soraya Bravo, Humberto Prieto and Andréa Miyasaka Almeida. 7th International Rosaceae Genomics Conference, Seattle, Washington, USA, June 24-26, 2014. (2014)
• Paola Barba, Aurélie Chague, Veronique Decroocq, Jean-Philippe Eyquard, Christian Montes, Humberto Prieto, Daniela Quiroz, Julia Rubio, Evelyn Sanchez and David Tricon. Plum pox virus – Prunus spp. interaction: the small RNA talk. January 2017 · XXV Plant and Animal Genome Conference - Fruits and Nuts Workshop., San Diego, Jan 14th. Pacific Salon 4.; 01/2017 (2017)
• Evelyn Sánchez, Karin Rothkegel, Christian Montes, Paola Andrade, Pablo Cid, Andrea M. Almeida and Humberto Prieto. Dormancy-associated microRNAs in sweet cherry (Prunus avium L.): a first draft. January 2017 · XXV Plant and Animal Genome Conference - San Diego, Jan 14th - 18th. Poster 1224 (2017)

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